Effects of Potato Spindle Tuber Viroid on Tomato Gene Expression

Hybridization: PSTVd-MM

Hybridization protocol
Hybridization solutionN/A
Solution concentrationN/A
Blocking agentN/A
Wash procedureGeneChips were washed and stained in the Affymetrix Fluidics Station 400
Labeled Target Quantity10 ug of cRNA
Hybridization Time16 hr
Hybridization VolumeN/A
Hybridization Temperature45C
Hybridization InstrumentN/A

Scan information
Scan hardwareAffymetrix GeneChip Scanner 3000 7G
scan softwareAffymetrix GeneChip Command Console software

Image analysis information
SoftwareAffymetrix GeneChip Operating Software (GCOS)
Analysis algorithmMAS 5 software

Normalization information
Normalization softwareMAS 5
Normalization algorithmN/A
Normalization strategyScaling