Tomato wild type, vector control and transgenic lines expressing InsP 5-ptase: mature fruits, root tips, and leaves
Hybridization: Vector control (root tips) |
Hybridization protocol |
Hybridization solution | N/A |
Solution concentration | N/A |
Blocking agent | N/A |
Wash procedure | GeneChips were washed and stained in the Affymetrix Fluidics Station 400 |
Labeled Target Quantity | 10 ug of cRNA |
Hybridization Time | 16 hr |
Hybridization Volume | N/A |
Hybridization Temperature | 45C |
Hybridization Instrument | N/A |
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Scan information |
Scan hardware | Affymetrix GeneChip Scanner 3000 7G |
scan software | Affymetrix GeneChip Command Console software |
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Image analysis information |
Software | Affymetrix GeneChip Operating Software (GCOS) |
Analysis algorithm | MAS 5 software |
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Normalization information |
Normalization software | RMA |
Normalization algorithm | N/A |
Normalization strategy | Total array |
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