Gene expression profiling of infection of tomato by Phytophthora infestans in the field

Hybridization: Time course 12hI-12hNI

Hybridization protocol
Hybridization solutionCorning Universal Hybridization Solution (catalog #40090, Corning Inc.)
Solution concentration Unknown due to Corning patent
Blocking agentUnknown due to Corning patent
Wash procedure1) Wash arrays in 1X SSC/0.2% SDS for 10 minutes at 43 C (in the dark).
2) Wash arrays in 0.1X SSC/0.2% SDS for 10 minutes at room temperature (in the dark, with shaking).
3) Wash arrays in 0.1X SSC for 10 minutes at room temperature (in the dark, with shaking).
Labeled Target QuantityAll (see instructions in Invitrogen labeling kit)
Hybridization Time12 - 16 hours
Hybridization Volume65 ul
Hybridization Temperature43C
Hybridization InstrumentCorning microarray hybridization chamber in 43C incubator

Scan information
Scan hardwareAxon
scan softwareGenepix

Image analysis information
SoftwareGenepix pro v 4.1
Analysis algorithm1) Cy5=red, Cy3=green
Default setting for image analysis used

Normalization information
Normalization softwareMidas
Normalization algorithmN/A
Normalization strategyLowess