Matt's wild cherry tomato vs. Phytophthora infestans
Hybridization: Exp 12h |
| Hybridization protocol |
| Hybridization solution | 0.1%SDS, 25% Formamide, 5xSSC |
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| Solution concentration | 0.1%SDS, 25% Formamide, 5xSSC |
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| Blocking agent | Liquid block (Amersham) |
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| Wash procedure | 5mins 42C 2x SSC, 0.1% SDS, 10mins RT 0.1x SSC, 0.1% SDS, 4mins RT 0.1x SSC |
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| Labeled Target Quantity | 50g |
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| Hybridization Time | 14hours |
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| Hybridization Volume | 45 ul |
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| Hybridization Temperature | 42C |
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| Hybridization Instrument | Corning microarray hybridization chamber in 42C incubator |
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| Scan information |
| Scan hardware | Axon |
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| scan software | Genepix |
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| Image analysis information |
| Software | Genepix pro v 4.1 |
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| Analysis algorithm | 1) Cy5=red, Cy3=green
Default setting for image analysis used |
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| Normalization information |
| Normalization software | marray package under R |
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| Normalization algorithm | Print-tip specific Lowess Normalization |
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| Normalization strategy | Total Array |
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