IL carotenoid

Hybridization: 4-3/M82

Hybridization protocol
Hybridization solutionSDS Genisphere Hybridization Solution
Solution concentration0.25m NaPO4, 4.5%SDS, 1mm EDTA, 1x SSC, 2x Denhardt's Solution
Blocking agentssDNA and Oligo dT
Wash procedure2x15 minutes
1) 2x SSC + 0.2% SDS at 55 C.
2) 2x SSC at RT.
3) 0.2x SSC at RT (+ 0.5mM DTT)
Labeled Target Quantity6 ul labeled cDNA
Hybridization Time(1) 14 hours (2) 2 hours
Hybridization Volume65 ul
Hybridization Temperature60 C / 55 C for secondary hyb
Hybridization InstrumentCorning hybridization chambers in water bath

Scan information
Scan hardwareScan Array 5000
scan softwareScan Array ver 3.1

Image analysis information
SoftwareGeneSight
Analysis algorithmN/A

Normalization information
Normalization softwareGeneSight
Normalization algorithmN/A
Normalization strategyN/A