Tomato introgression line transcript analysis

Hybridization: IL4-4/WTpooled

Hybridization protocol
Hybridization solution0.1%SDS, 25% Formamide, 5xSSC
Solution concentration0.1%SDS, 25% Formamide, 5xSSC
Blocking agentLiquid block (Amersham)
Wash procedure5mins 42C 2x SSC, 0.1% SDS, 10mins RT 0.1x SSC, 0.1% SDS, 4mins RT 0.1x SSC
Labeled Target Quantity50g
Hybridization Time14hours
Hybridization Volume45 ul
Hybridization Temperature42C
Hybridization InstrumentCorning microarray hybridization chamber in 42C incubator

Scan information
Scan hardwareAffymetrix 428 Array Scanner
scan softwareAffymetrix 428 array reader

Image analysis information
SoftwareGenepix pro v 4.1
Analysis algorithm1) Cy5=red, Cy3=green
Default setting for image analysis used

Normalization information
Normalization softwareExcel Spreadsheets used to calculate total signal
Normalization algorithmN/A
Normalization strategyTotal array